Welcome to MSH!

Tri-State has Gone Green!

In an effort to be more green we have decided not to print handouts again this year. If you would like a copy of the presentation prior to the event, please see the linked documents below. You are more than welcome to print a copy and bring it with you to the presentation.

PDF Documents Coming Soon!

Wednesday, May 8th

3:00-4:00 QC with QRs - More and Better Quality Feedback Using 2-D Barcodes


Providing feedback on quality issues in the laboratory is a cornerstone to process improvement and a key regulatory requirement, but many obstacles prevent timely, consistent recording of errors. Many lab staff members are happy to provide feedback, however a workstation geared toward histology workflow may not be well-suited to typing in notes. By using barcode scanners as text entry devices we were able to increase the amount of quality documentation collected, and use the information to implement strategies to resolve issues.

3:00-4:00 Approach to Interpretation of Gastrointestinal Biopsies in Veterinary Medicine

Jesse M. Hostetter, DVM, PhD, DACVP

The goal of this seminar will be to provide an overview of the approach for interpretation of gastrointestinal biopsies in veterinary medicine. This will include a general comparison of full thickness intestinal biopsies and endoscopic biopsies, with advantages and limitations of both approach. For stomach, small intestine, and colon a brief overview of normal microscopic features will be reviewed. Common microscopic changes in each segment of the gastrointestinal tract will be discussed and a standard scoring system for determining severity will be reviewed. To conclude the seminar unknown intestinal biopsy cases will be presented.

4:00-5:00 The Diagnosis of the Prion Diseases of Animals

Justin Greenlee, DVM, PhD, Diplomate ACVP

The transmissible spongiform encephalopathies (TSE’s) or prion diseases are fatal neurodegenerative diseases caused by accumulation of a misfolded form of the prion protein called PrPSc. Historically, these diseases were diagnosed microscopically by examining HE stained brain sections for the presence of neuron degeneration, gliosis, and spongiform change, which can appear as either small round empty spaces in the neuropil or vacuoles present within neuron cell bodies. Identification of the TSE’s as diseases of the prion protein opened new avenues for antibody based diagnostic tests such as western blot and immunohistochemistry. Immunohistochemistry is considered the gold standard in the diagnosis of prion diseases; however, much effort has been put into new diagnostic or screening methods that can be performed on live animals. In our lab we have developed two screening technologies for prion diseases that utilize examination of the retinas of live animals: Optical coherence tomography that measures retinal thickness and electroretinograms that measures the electrical impulses generated in the eye as visual stimulus is converted into a nerve signal. Both of these techniques identify animals incubating prion diseases prior the onset of clinical signs. Microscopic evaluation of retinal tissues and the use of tissue specific cell markers were instrumental in the process of validating these techniques for the identification of prion diseases in live animals.

4:00-5:00 Evaluating data: What do we really know?

Eric R. Burrough, DVM, PhD, DACVP

What do we think we know and why do we believe it to be true? To quote the British philosopher Bertrand Russell, “It is not what the man of science believes that distinguishes him, but how and why he believes it.” A certain amount of scientific due diligence should be applied to all laboratory diagnostic and peer-reviewed data before it is accepted as fact. Inattentional blindness, inductive reasoning, and conformational bias are realities that must be considered and can impact all datasets. Inarguably you can only find what you look for, and you only recognize what you know, but how much data is either inappropriately interpreted or not even accounted for because the collector of the data simply did not know?.

5:00-6:00 Waste Management for the Histology Lab

James H. Taylor, MBA, CSSBB, CPM**Sponsored by BR Instruments Corporation

The purpose of this seminar is to instruct the attendees on waste management for the histology lab. All histology labs use organic solvents such as xylene, xylene substitutes, alcohols, and formalin.  Some of these solvents are expensive to purchase and fluctuate with the price of energy needs.  The costs for the disposal of these solvents have become more expensive than the purchase costs. This seminar will provide instruction on how to identify and collect solvents for recycling from the processor and stain lines. The attendee will learn what solvents can be recycled.  They will also learn the why some solvent mixtures should never be recycled.  They will also learn QC procedures for the laboratory. This seminar will provide the fundamentals for starting a solvent recycling program in a pathology department.  The attendees will become educated in the different recycling processes and will be able to make informed decisions when purchasing recyclers.

5:00-6:00 The Trouble with Bovine Tuberculosis

Mitch Palmer, DVM, PhD

Bovine tuberculosis once caused one-third of all human tuberculosis cases in the United States. Eradication efforts led by the USDA, combined with mandatory milk pasteurization have greatly decreased the prevalence of the disease in both humans and cattle; however, eradication from the US cattle population has remained elusive. In spite of the ancient origins of bovine tuberculosis, much about the pathogenesis remains unclear. It is evident that there is a need for understanding pathogenesis at the level of the granuloma; the stereotypical lesion of tuberculosis. Within the granuloma, host meets pathogen and the outcome of infection is determined. Insights into the frontline of host-pathogen interactions will aid our understanding of this ancient disease.

Thursday, May 9th

8:30-12:00 Introduction to Tissue Identification and Pathology 

**Handouts for this presentation available by request from presenter

Ada Feldman, MS, HT/HTL(ASCP) **Sponsored by Sakura

Pathology involves the study of healthy and diseased tissues. Therefore an applicable tissue identification course would include learning the appearance of both normal and abnormal tissue. This course will cover the tissues commonly received in a clinical pathology laboratory. It begins with an overview of the four basic tissue types: epithelial, muscle, nervous and connective tissues. This will be followed with identification of normal and respective pathologies of skin, gastrointestinal, urinary, lymphatic, respiratory and reproductive systems. H&E, special stains and IHC will be represented. The material introduced will assist technicians in evaluating the quality of their stained tissue sect.

1:00-2:30 Using DiSC to Decode and Diminish Conflict

Sharon Kneebone, BA, CAE, IOM **Sponsored by NSH

Everyone is not like you! As individuals we share a majority of personal and cultural values. There are subtle variations in individual values and preferences which can lead to BIG differences in points of view in how we lead, how we communicate, and how we get work done. These variations can lead to misunderstandings and conflict. DiSC is a behavior assessment tool based on the DiSC theory of psychologist William Moulton Marston, which centers of four different behavioral traits:  dominance, influence, steadiness, and conscientiousness. No one trait is considered either good or bad, just different. DiSC has been successfully used to provide a framework for understanding and highlighting individual strengths as well as addressing potential blind spots.  To understand others, we must first understand ourselves and then seek to understand.  Participants will learn about the four personality types and complete a brief self-evaluation to learn about and understand their preferences.  In addition to lecture, participants will be provided with scenarios, break into small groups, and given the opportunity to apply DiSC on how to find a path forward that is neither emotional nor judgmental.

1:00-2:30 Next Generation IHC  

Jacob Wilson, BS **Sponsored by Cell Marque/Millipore Sigma

The majority of the antibodies that are currently run in clinical labs have been around for quite a while. We also know that research is ongoing to uncover new information about cancer; so, where has all the research led us? Many of the recent antibodies to hit the market do not have a completely novel utility, in fact a good number of them can be considered the second generation of testing. However, that is the where the benefit lies. Do you have some antibodies that are not as sensitive as you would prefer, what about some lacking specificity? This workshop is designed to inform the lab of some of the recent antibody developments and how they improve patient care.

3:00-4:30 Histogonomics-Ergonomics and Injuries in the Histology Laby

Brad Flowers, BS **Sponsored by Cancer Diagnostics

Work-related musculoskeletal disorders are the single largest job-related injury and illness problem in the United States and account for nearly 1/3 of all occupational occurrences reported.  The field of Histology is inundated with repetitive motion activities on a daily basis and your lab environment may be a contributing factor for triggering injuries.  This course discusses each of the most common injuries occurring in a Histology Lab, ways to treat these injuries, and how to prevent them from occurring.  The goal is to increase awareness so that your lab is more efficient by not having lost time with a workplace injury and avoiding the discomfort and pain associated with these injuries.  Today’s labs are busier and more demanding than ever and we must consider making changes to provide better outcomes.  The best intentions do not mitigate loss or alleviate disaster, preparation does.  Therefore, “An ounce of prevention is worth a pound of cure.”

3:00-4:30 Strategies to Identify and Eliminate Background Interference in IHC and IF Tissue Section Application

Craig Pow, PhD **Sponsored by Vector Labs, part of Maravai LifeSciences

Background signal in defined antigen staining applications is often encountered. This unwanted staining presents challenges in assay validation and interpretation of specific staining, and manifests false positives. To circumvent these confounding and problematic situations, timely and accurate identification of the background source, in combination with significant reduction or elimination of background using appropriate measures, is paramount to achieving meaningful, reliable data. This seminar will provide approaches to pinpoint the origin(s) of background signal, as well as strategies that can be implemented to solve the background problem. Techniques for both immunohistochemistry and immunofluorescence workflows will be addressed.

Friday, May 10th

8:30-10:00 Troubleshooting – Behind the Scenes

**Handouts for this presentation available by request from presenter

Ada Feldman, MS, HT/HTL(ASCP) **Sponsored by Sakura

Correct H&E staining is dependent upon the binding of the dye molecule to the tissue. If the chemistry of either of these is changed, pairing does not occur and abnormal staining is the result. This workshop will go ‘behind the scenes’ to show what in processing, staining solutions or the staining protocol can interfere in the pairing of the hematoxylin and/or eosin stain to the tissue.

8:30-10:00 Multiplex Immunohistochemistry Assays: Best Practices, Techniques, and Troubleshooting

Victoria Duckworth, MS **Sponsored by Perkin Elmer/Akoya Team

As we continue to discover and probe the crucial implications of cellular interactions involved in the diagnosis, prognosis, and treatment of disease, multiplex IHC assays have become the foremost tool in researching in vitro systems biology. The development, optimization, and validation of a multiplex IHC assay requires planning and forethought. Some of the most important factors for success in multiplexing involve following best practices for pre-analytical workflows, robust antibody validation, careful panel design, appropriate controls, and the choice of detection chemistry or technologies. This includes considerations for manual versus automated staining, digital pathology and image analysis, and emerging technologies. Additionally, an understanding of the common pitfalls and challenges associated with the different techniques, as well as methods available for troubleshooting each, is key to successful optimization and validation

8:30-12:00 Microarray 101: The Basicrs

Colleen Forster, HT(ASCP)QIHC, BSBA, and Lori Holm, HT(ASCP)**Sponsored by Newcomer Supply and Simport

With the rapid interest and need for new biomarkers, the increasing cost of high complex testing, budget cuts, workforce shortages, the need to maintain quality, the tissue microarray (TMA) may offer some help. This workshop will concentrate on several key aspects for good TMA production: purpose, design, tissue selection, and annotation of slides, TMA map design, sectioning and staining. Participants will get the chance to construct a simple TMA using a microarray mold.

10:30-12:00 Basic Repair and Equipment Maintenance of the Equipment in your Lab

Matt Mincer **Sponsored by Tech One Biomedical Servic

Let’s face it; life in the lab is stressful enough without having to worry whether your equipment will work when you get there. Over the years we have found that many problems can be resolved quite easily and with no technical background. This workshop is designed to help the histotech resolve many of these issues without having to “call the service guys”. We will also discuss maintenance and some “do’s” and “don’ts” that will help prevent future problems. Hopefully, you will find the information helpful and use it to avoid potential needless service calls.

10:30-12:00 Tissue Sample Handling & Preparation for Molecular Pathology

Robert Jacox, MBA and Jennifer Freeland, MS, HTL(ASCP) **Sponsored by Thermo Fisher Scientific

In the age of molecular pathology, tissue sample quality has become redefined. Tissue sample handling and preparation should be optimized with the end result in mind. This seminar is designed to highlight best practices from tissue sample collection, through tissue processing & microtomy, and biomolecule extraction. Whether the end molecular assay is NGS, IHC, proteomics, or PCR, optimal tissue preparation is important. Quality control test methods, workflows, and comparative test results will also be reviewed.
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